LIQUID SCINTILLATION Zinsser Analytic.doc

1、LIQUID SCINTILLATIONLiquid ScintillationGermany:ZINSSER ANALYTIC GMBHEschborner Landstrasse 135 D-60489 FrankfurtTel.: +49(0)69 / 78 91 06-0Fax: +49(0)69 / 78 91 06-80Email: infozinsser- Web: www.zinsser-USA:ZINSSER NA Inc.19145 Parthenia Street - Suite C Northridge CA 91324Tel.: +1 (0)818 / 341 29

2、06Fax: +1 (0)818 / 341 29 06Email: info Web: Impressum:Zinsser Analytic GmbH, Eschborner Landstrasse 135, D-60489 Frankfurt Text and Layout: Werner Zinsser, Anja EstlerPrinter: Baier Digitaldruck GmbH, HeidelbergAll material in this catalogue is protected by copyright and reproduction, publishing or

3、 storage of any text or illustration by any means whatsoever is prohibited, unless authorised by Zinsser Analytic in writing.We reserve the right to change prices or product specifications without prior notice.All orders are subject to our written terms and conditions which are available on request.

4、ContentMany advances have been made over the years in scintillation counters and in cocktail development. The Zinsser Analytic range of safe scintillators is formulated using a solvent which is non toxic, biodegradable, odourless and has a flash point above 150C. As well as being highly effective sc

5、intillators they are also pleasant to use and safe for storage and transport. Our range also includes liquid scintillators for flow cell applications. TheQuickszint flow scintillators are for mu lated to count HPLC gradients such as acetonitrile/ water as well as buffer solutions and pure solvents.Z

6、insser Analytic have manufactured Polyvials (20 ml) and MINIS plastic counting vials for many years. We also supply high quality glass vials from Wheaton Science Products and a Scintillation Counter from HITACHI-ALOKA.Scintillation CounterAccuFLEX LSC-80005AccuFLEX LSC-LB76OxidizersOX-5017OX-7018Rob

7、ox9 Oxidizer Accessories10 Harvester9611Counting Vials, Plastics & GlassPolyvials13Mini Vials16Glass Scintillation Vials and Caps18Racks and Trays20Scintillation CocktailsLiquid Scintillation Selection21Safe Scintillators25Flow Scintillators29Special Scintillators30Dispensing Scintillators31Terms &

8、Conditions 333013013雀S甜甜咄忱S提别130,133601301336()13013uau13Low Level Scintillation CounterHITACHI-ALOKA LSC-8000liquid scintillation counterAccuFLEX LSC-8000 ensures accuracy and flexibility for any basic liquid scintillation measurement in research and environmental laboratories. Quantification of al

9、l the beta emitters (with optional alpha/beta separation)is performed using 4000 channels and optimized energy ranges. Pre-set counting windows or Auto Isotope Selection offers any result unitsin counts, cpm, dpm or Bq.LSC-8000 can be used with either 20 ml, 8 ml,6 ml glass or plastic vials or micro

10、 tubes, with a 408 sample loading capacity in its racks-based samples changer.Accurate measurements are provided with External Standard Channel Ratio, Self Constant Channel Ratio methods and other advanced features such as Chemical Luminescence, Color Quenching Corrections and Anti-Static Function.D

11、ata reliability is fully supported by a System Performance Evaluation, including History Review and Security locks, in a GLP mode, with 21 CFR Part 11 included as a standard feature.Windows based user friendly software andbuilt-in PC with touch panel color LCD and various output interfaces gives all

12、 the comfort required on a modern, easy-to-use standard liquid scintillation counter.Technical Data:Efficiencies:3H : 60%, 14C : 95% with unquenched sealed standards Sample Changer:up to 408 samples, using racksAnalyzer:4000 channels, up to 3 counting windows, pre-set windows for 3H, 14C, 32P, 125I,

13、 32P Cerenkov, 3H + 14C, 3H + 14C + 32PBackground: 15cpm depending on vial size and type Dimensions:960 x 600 x 850 mm (W x H x D)Weight:196 kgCatalogue No.300060009HITACHI-ALOKA LB-7low level, large volume liquid scintillation counterLB-7 is a large volume scintillation counter for low level measur

14、ement of beta isotopes. It can be used with either 20 ml or 145 ml scintillation vials. The background level is much lower than with any other scintillationcounter, due to the large passive lead shielding and active anti-coincidence shielding. The detection threshold for tritium is better than0.2 Bq

15、/litre in 24 hours counting time.The LB-7 is easy to use, with the operation and the definition of the measuring window appearing on the VDU. Two multi-channel analysers enable a spectral analysis to be produced on the VDU, which can be plotted out and saved to disk.The instrument provides automatic

16、 quench correction through ESCR and SCCR, an antistatic device, a monitor for chemiluminescence and photoluminescence and isotope half life correction.Technical Data:Sensitivity:for 3H 0.17 Bq/litreSample changer:cooled, for 20 samples in 145 ml, 100 ml, 20 ml or 8 ml vials with adaptors Detector:5

17、selected photo-multipliers tubes: 3 for the measuring chamber, 2 for theactive guard counterSpectrometer:variable window selection, 3 independent measuring channelsBackground: 2.5 cpmDimensions:1230 x 810 x 1600 mm (W x D x H)Weight:800 kgCatalogue No.30007700Sample OxidizersOX-501The Compact Automa

18、tic OxidizerThe OX-501 is for quench free sample preparationof biological samples which are labelled with 3H or 14C. If the samples are duallabelled, the 3H and 14C are automatically separated.The OX-501 works on the principle of catalytic combustion. The sample is combusted at 900C in a flow of oxy

19、gen within the horizontal combustion tube. The high combustion temperature guarantees the quick and complete combustionof aqueous solutions, blood, wet filters and dampsurface swabs.OX-501 offers the user the following advantages: Quench free samples Easy operation High reliabil ity Compact design E

20、conomically pricedIt is a fully automatic system with electronic controls for gas flow, scintillator volume and combustion time.OX-501 oxidizers are currently used in: The pharmaceutical industry Quality control Research laboratories Environmental researchTechnical Data:Recovery:for 3H and 14C bette

21、r than 98%Reproducibility: 1%Memory effect:negligible, less than50 dpm for 14C after repeated combustionof 100,000 dpm samplesDimensions:520 x 380 x 560 mm(W x D x H)Catalogue No.36013000OX-701Proven Oxidizer Techniques with Automated Sample & Vial Handling Combusts wet or dry samples and liquids Co

22、mbusts samples up to 300 mg i.e. faeces ortissue homogenates or 0.50 ml whole blood Single or duallabelled samples, 14C & 3H A sample presentation method that preventsresidue build-up common to other systems No sample preparation required Ideal for soil or high ash content materials and fatty sample

23、s Combustion temperature of 900C Rapid start, less than 15 minutes from cold Catalytic oxidation ensures removal of quenching agents such as SO2, NO2 and others Load 12, 14C samples and walk away when working in the 1-12 MODE Easily serviced by the user at their facility orthe unit can be quickly di

24、sassembled for factory serviceTechnical Data:Recovery:for 3H and 14C better than 98% Continuous sample combustion in the 99 MODE Reproducibility: 1%and stat determinations in the 00 MODE Some existing OX-501 units can be retrofitted Recovery 98% Reproducibility 1% Low memory, less than 50dpm above b

25、ackground on samples up to 100,000 dpmMemory effect:negligible, less than50 dpm for 14C after repeated combustionof 100,000 dpm samplesDimensions:965 x 356 x 534 mm(W x D x H)Catalogue No.36014000Sample Oxidizer AccessoriesRoboxFully automated sample preparation & oxidationRobox is our system for fu

26、lly automatic quench free preparation of samples for liquid scintillation counting.The sample preparation method is made easier due to the use of an intelligent robot, which enables the system to prepare 120 samples of 14C or dual labelled samples in only 8 hours.Its multi-user facility allows the a

27、ddition of extra samples during the work sequence.Robox consists of a robot with a linear drive and an automatic oxidiser OX-501, which works on the principle of catalytic oxidation. The samples are laid out in a tray and Robox then does the rest.It transfers the samples to the oxidiser, transports

28、the empty scintillation vials to the oxidiser, collects them after they have been filled with scintillator and combustion product, seals & barcodes them and places them in a transport tray or rack ready for the scintillation counter.The whole work sequence is monitored bysensors and is computer cont

29、rolled.The information supplied by the Robox is: Sample number Sample ID Date and time Temperature Total combustion listRobox monitors all process parameters as combustion time, oxigen flow, combustion temperature and sample number.Robox helps you save on costs:Costs are in comparison with manual sa

30、mple preparation reduced by half due to the large number of samples which can be processed automatically in a single run.10Quartz combustion tube for OX300, 501 & 701Without catalyst, 1 tubeCatalogue No.36013010With catalyst, 1 tubeCatalogue No.36013011Tips for OX 300, 400, 501Tips made from polyeth

31、ylene for 3H and 14Cspouts of Harvey Oxidizer. Pack Size 1000 tipsCatalogue No.36013021O-RingsSpecial O-Rings made from solvent resistant plastic for 3H and 14C spouts.Pack Size 10 O-RingsCatalogue No.36013022Quartz LadlesQuartz ladles with ground joint for use with Harvey Oxidizers.Pack Size 1 ladl

32、eCatalogue No.36013012Quartz WoolPack of 100gCatalogue No.36013004Catalyst 1Copper Oxide, packed in plastic bottles.Catalogue No.36013001Catalyst 2Silver Vanadium, packed in plastic bottles.Catalogue No.36013002Catalyst 3A special, fine mesh net made from platinumgauze.Catalogue No.36013003Sample Bo

33、atsSample boats made from porcelain for storage, weighing out and combustion of samples.Pack Size 10 boatsCatalogue No.36013013Harvester96Harvester96The Harvester96 is cost effective, simple to use and designed to last for decades. Tomtec Life Science has been servicing the laboratory automation com

34、munity for over twenty of those years. Based on a highly reliable and eloquent design, the Harvetser96 is a rugged cost effective bench-top lab instrument, designedto harvest biologics from a 96 well microplate or microtubes onto a glass fiber filter map in a small bench-top footprint.The Harvester9

35、6 is designed to address a wide range of Pharma, BioPharma and laboratory work-flows such as: Cell proliferation assays (titrated thymidine uptake) Scintillation Counter Cytotoxicity assays luminescent assay reader Receptor binding assay luminescent assay reader Nucleic acid degradation spectrophoto

36、meter assay reader Trichloroacetic acid preparations microscopy Cell wash station for adherent cell assaysHarvester96ManualAutomaticbench foot-print12“ wide x 24“ deep12“ wide x 24“ deepheightclosed 16“ / open 22“closed 16“ / open 22“weight50lb50lbpower120w120wvacuum connections12reagent connections

37、12reagent bottlesone 9 liter bottle with thread on feed cap pressure connection (5 psi)two 9 litre bottles with thread on feed cap pressure connection (5 psi)vacuum Autotrapsone 11.5 litre trap with gage andhigh level float controltwo 11.5 litre traps gage and highlevel float controlPlastic Counting

38、 VialsZINSSER POLYVIALS19Plastic counting vials have superseded low potassium glass counting vials for several reasons. Their low price makes it possible to use them once and then to dispose of them, taking away the need for cleaning as is required with the more expensive glass vials. Further advant

39、ages are the low background rates which are less than 5-7 cpm and the higher UV transmission between 360 and 400 nm.precision- moulded neck uniform wall- thicknessThe dimensions of these counting vials are the same as those for all normal vials for liquid scintillation counting techniques. However,

40、there are marked differences from those offered by other manufacturers. One of the main differences is the type of raw materials used in production. Zinsser Analytic uses only high density rigid polyethylene which has no impurities in it (which may result in quenching). Many cheap plastic vialsseaml

41、ess baseno thin areasare made with re-ground materials, which raises the background rate and the risk of quenching. These cheap vials tend to expand when used with solvents such as xylene or toluene and may become stuck in the counting chambers.Further differences in quality occur through production

42、 techniques. Most of the lower quality and cheaper counting vials are produced by extrusion blow moulding. It is easy to identify such vials by noting their uneven wall thickness, the seam on the bottom and the rough edges round the neck of the vial. Micro fine cracks can appear in the bottom seam a

43、nd ridges can appear at the neck so that the vial can never be sealed properly. This makes the use of such vials in liquid scintillation counters very risky on three counts:1. a danger of contamination because of the possibility that the bottom seam may not be sealed properly.2. a risk of mechanical

44、 jamming because of ridges in the bottom of the vial.3. poor cap sealing because of ridges in the neck or an in completely formed neck.The uneven wall thickness leads to a higher solvent loss by diffusion and greater risk of error, if the sample is not measured straight after itspreparation. Other d

45、isadvantages of extrusion blow moulded vials are the great deviations inthe dimensions of the diameter and height, which can result in mechanical difficulties in liquid scintillation counters.High quality scintillation counting vials such as Polyvials are injection blow moulded. This is a two step process. In the first step the neck area is moulded with a preformer. The preformer is h